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產品名稱：EosinophilChemotacticFactor(ECF) ELISA Kit
產品型號：
產品廠商：國內供應1
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簡單介紹：

EosinophilChemotacticFactor(ECF) ELISA Kit

詳情介紹：

Product details
Product details
Purpose	The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of ECF in bovine serum, plasma and other biological fluids.
Sample Type	Biological Fluids, Plasma, Serum
Analytical Method	Quantitative
Detection Method	Colorimetric
Specificity	This assay has high sensitivity and excellent specificity for detection of this index.
Cross-Reactivity (Details)	No significant cross-reactivity or interference between this index and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between this index and all the analogues, therefore, cross reaction may still exist.
Sensitivity	28.6 pg/mL
Components	Pre-coated, ready to use 96-well strip plate Standard (freeze dried) Standard Diluent Detection Reagent A Detection Reagent B Assay Diluent A Assay Diluent B TMB Stop Solution Wash Buffer (30X) Plate sealer for 96 wells Instruction manual
Material not included	Microplate reader with 450 ± 10nm filter. Precision single or multi-channel pipettes and disposable tips. Eppendorf Tubes for diluting samples. Deionized or distilled water. Absorbent paper for blotting the microtiter plate. Container for Wash Solution.

Target details
Target details
Background	Synonyms: SCYA11, CCL11, Eotaxin 1, Eosinophil Chemotactic Protein 1, Chemokine C-C-Motif Ligand 11,
Gene ID	404072
UniProt	Q9TTS6

Application Details
Application Details
Sample Volume	100 μL
Assay Time	1 - 4.5 h
Plate	Pre-coated
Protocol	1. Prepare all reagents, samples and standards 2. Add 100 μL standard or sample to each well. Incubate 2 hours at 37 °C 3. Aspirate and add 100 μL prepared Detection Reagent A. Incubate 1 hour at 37 °C 4. Aspirate and wash 3 times 5. Add 100 μL prepared Detection Reagent B. Incubate 30 minutes at 37 °C 6. Aspirate and wash 5 times 7. Add 90 μL Substrate Solution. Incubate 15-25 minutes at 37 °C 8. Add 50 μL Stop Solution. Read at 450nm immediately.
Assay Procedure	The microtiter plate provided in this kit has been pre-coated with an antibody specific to the index. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to the index. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain the index, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of the index in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Assay Precision	Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-assay: CV<10% Inter-assay: CV<12%
Restrictions	For Research Use only

Handling
Handling
Precaution of Use	The Stop Solution suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.
Handling Advice	The stability of ELISA Kit is determined by the loss rate of activity. The loss rate of this kit is less than 5?% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Storage	4 °C,-20 °C
Storage Comment	The Assay Plate, Standard, Detection Reagent A and Detection Reagent B should be stored at -20°C upon being received. After receiving the kit , Substrate should be always stored at 4°C.Other reagents are kept according to the labels on vials. But for long term storage, please keep the whole kit at -20°C. The unused strips should be kept in a sealed bag with the desiccant provided to minimize exposure to damp air. The test kit may be used throughout the expiration date of the kit (six months from the date of manufacture). Opened test kits will remain stable until the expiring date shown, provided it is stored as prescribed above.
Expiry Date	12 months

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