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  • 產(chǎn)品名稱：Goatanti-HumanIg(LightChain),(Chainlambda)Antibody(Biotin)

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  • 產(chǎn)品廠商：Acris-antibodies
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簡單介紹：
Goatanti-HumanIg(LightChain),(Chainlambda)Antibody(Biotin)
詳情介紹：
Immunogen Human lambda (lambda) light chain
Fragment F(ab')2 fragment
Characteristics Molar Ratio: 10-20 BAC molecules per Goat IgG Fab molecule.
Purification Immunoaffinity chromatography.
Alternative Name Immunoglobulin
Research Area Secondary Antibodies, Virology
Application Notes Suitable for Immunoblotting, ELISA, Immunohistochemistry, immunomicroscopy as well asother antibody based assays using streptavidin or avidin conjugates requiring extremelylow background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titerand specificity. Recommended Dilutions: This product has been assayed against 1.0?μg of Human IgG in astandard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS(2,2'-azino-bis-[3-ethylbenthiazoline-6- sulfonic acid]) as a substrate for 30?minutes atroom temperature. A working dilution of 1: 4,000 to 1: 20,000 of the reconstitutionconcentration is suggested for this product.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Reconstitution Restore with 1.0?mL of deionized water (or equivalent).
Concentration 1.0 mg/mL (by UV absorbance at 280 nm)
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride,?pH 7.2 with 10?mg/mL Bovine Serum Albumin (BSA) IgG and Protease free as stabilizer and 0.01?% (w/v) Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing. Dilute only prior to immediate use
Storage 4 °C/-20 °C
Storage Comment Store vial at 2-8 °C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20 °C or below. Centrifuge product if not completely clear after standing at room temperature. This antibody is stable for one month at 2-8 °C as an undiluted liquid.
Background publications Bayer, Wilchek: "Protein biotinylation." in: Methods in enzymology, Vol. 184, pp. 138-60, 1990 (PubMed).