產品詳情

  • 產品名稱：HumanSemaphorin4D/SEMA4D/CD100HumanCellsTransfectedLysate(positivecontrol)(dena

  • 產品型號：
  • 產品廠商：SinoBiological
  • 產品文檔：
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簡單介紹：
HumanSemaphorin4D/SEMA4D/CD100HumanCellsTransfectedLysate(positivecontrol)(denatured)
詳情介紹：
Characteristics denatured
Human Cells transfected lysate in which Human SEMA4D / CD100 has been over-expressed. The whole cell lysate is provided in 1X Sample Buffer.

Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation.
Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Microplate Standard assay).

The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5 % glycerol, 1 % sodium dodecylsulfate, 0.01 % bromophenol blue) containing 5 % b-mercaptoethanol, and lyophilized.
Sterility Non-sterile
Alternative Name SEMA4D
Application Notes WB: Use at an assay dependent dilution.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Protocol 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.
2. Re-dissolve the pellet using 200 μL pure water and boiled for 2-5 min.
3. Store it at -80 °C.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Reconstitute in pure water
Buffer Modified RIPA Lysis Buffer: 50 mM Tris-HCl?pH 7.4, 150 mM NaCl, 1?mM EDTA, 1?% Triton X-100, 0.1?% SDS, 1?% Sodium deoxycholate, 1?mM PMSF.
Handling Advice Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.
Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Storage 4 °C/-80 °C
Storage Comment Store at 4°C. After re-dissolution, aliquot and store at -80°C.
Expiry Date 12 months