產(chǎn)品詳情
簡單介紹:
HumanIgGisotypecontrol(HRP)
詳情介紹:
Isotype | IgG |
Fragment | F(ab')2 fragement |
Characteristics |
Human IgG protein (Fab'2) (HRP) conjugate Source: Human serum Alternative Names: Immunoglobulin G protein (Human) (Fab'2) (HRP) Physical state: Clear, colorless liquid |
Purification | Human IgG protein (Fab'2) (HRP) was purified by delipidation, salt fractionation and ion exchange chromatography followed by papain digestion and dialysis. |
Background | Immunoglobulin G (IgG) are antibody molecules. Each IgG is composed of four peptide chains - two heavy chains and two light chains. Each IgG has two antigen s. Other Immunoglobulins may be described in terms of polymers with the IgG structure considered the monomer. The fragment antigen-binding (Fab fragment) is a region on an antibody that binds to antigens. It is composed of one constant and one variable domain of each of the heavy and the light chain. These domains shape the paratope - the antigen- - at the amino terminal end of the monomer. The two variable domains bind the epitope on their specific antigens. Treatment with pepsin produces an F(ab)'2 fragment. |
Research Area | Immunology, Secondary Antibodies |
Application Notes | Optimal working dilutions should be determined experimentally by the investigator. |
Restrictions | For Research Use only |
Format | Lyophilized |
Concentration | Lot specific |
Buffer | Lyophilized from 0.01 M Na1O4, pH 7.4, with 0.11 NaCl, 10 mg/mL BSA. Immunoglobulin and protease free. |
Handling Advice |
Avoid repeated freeze/thaw cycles. Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase. |
Storage | RT/-20 °C |
Storage Comment | Store at 4 °C until reconstitution. Following reconstitution aliquot and freeze at -20 °C for long term storage. |